Journal: Experimental Animals

Article Title: Hairless-knockout piglets generated using the clustered regularly interspaced short palindromic repeat/CRISPR-associated-9 exhibit abnormalities in the skin and thymus

doi: 10.1538/expanim.19-0018

Figure Lengend Snippet: Western blot analysis of hairless (HR) in eyelid skin samples of HR −/− and WT piglets. HR was detected using a polyclonal anti-Hr antibody. A polyclonal anti-actin antibody was used as a loading control.

Article Snippet: Membranes were blocked with 5% skimmed milk and incubated with the following primary antibodies overnight at 4°C: rabbit polyclonal anti-HR (1:1,000; Eterlife, Birmingham, UK) and goat polyclonal anti-actin (1:1,000; Santa CruzBiotechnology, Redwood, CA, USA).

Techniques: Western Blot, Control

Journal: Poultry Science

Article Title: Protective effect of nano-selenium on mercury-induced prehierarchical follicular atresia in laying hens

doi: 10.1016/j.psj.2022.102190

Figure Lengend Snippet: Effects of nano-Se addition on gene expressions of ERS pathway affected by Hg exposure in GCs. (A) Relative expression of PERK gene; (B) relative expression of ATF4 gene; (C) relative expression of CHOP gene; (D) relative expression of IRE1α gene; (E) relative expression of TRAF2 gene; (F) relative expression of ASK1 gene; (G) relative expression of ATF6 gene; (H) relative expression of Caspase-9 gene; (I) relative expression of Caspase-3 gene; (J) relative expression of Bax / Bcl-2 gene. Abbreviations: ERS, endoplasmic reticulum stress; GCs, granulosa cells; PERK, protein kinase RNA-like endoplasmic reticulum kinase; ATF4, activating transcription factor 4; CHOP, C/EBP homologous protein; IRE1α, inositol-requiring enzyme 1α; TRAF2, tumor necrosis factor-associated factor 2; ASK1, apoptosis signal-regulating kinase 1; ATF6, activating transcription factor 6; Bax, B-cell lymphoma-2 associate X . Values are the means ± SE (n = 8). a-c Means with different superscripts differ significantly among any groups ( P < 0.05).

Article Snippet: The antibodies of PERK (1:500, GB11510), IRE1α (1:500, GB11685), and ATF6 (1:500, GB11297) were purchased from Servicebio technology Co. Ltd.

Techniques: Expressing

Journal: Poultry Science

Article Title: Protective effect of nano-selenium on mercury-induced prehierarchical follicular atresia in laying hens

doi: 10.1016/j.psj.2022.102190

Figure Lengend Snippet: Effects of nano-Se addition on the key protein expressions of ERS pathway affected by Hg exposure in GCs. (A) The protein expressions of PERK, IRE1α and ATF6 were determined by Western blot; (B) relative protein expression of PERK; (C) relative protein expression of IRE1α; (D) relative protein expression of ATF6. Abbreviations: ERS, endoplasmic reticulum stress; GCs, granulosa cells; PERK, protein kinase RNA-like endoplasmic reticulum kinase; IRE1α, inositol-requiring enzyme 1α; ATF6, activating transcription factor 6 . Values are the means ± SE (n = 8). a-c Means with different superscripts differ significantly among any groups ( P < 0.05).

Article Snippet: The antibodies of PERK (1:500, GB11510), IRE1α (1:500, GB11685), and ATF6 (1:500, GB11297) were purchased from Servicebio technology Co. Ltd.

Techniques: Western Blot, Expressing